Correction: Evidence for Diversity in Transcriptional Profiles of Single Hematopoietic Stem Cells

نویسندگان

  • Carlos A Ramos
  • Teresa A Bowman
  • Nathan C Boles
  • Akil A Merchant
  • Yayun Zheng
  • Irma Parra
  • Suzanne A. W Fuqua
  • Chad A Shaw
  • Margaret A Goodell
چکیده

This buffer was also reported incorrectly in the Protocol S1. The second sentence under the heading ‘‘Global Single or minimal number Cell RT-PCR (GSC RT-PCR)’’ on the second page should be: ‘‘For 100 lL of lysis buffer, we combined 76 lL of RNase free water, 20 lL of first strand buffer 5x (250 mM Tris-HCl [pH 8.3], 375 mM KCl, 15 mM MgCl2), 1 lL of Prime RNase inhibitor (Brinkmann, Westbury, NY, USA), 1 lL RNase Guard (Promega, Madison, WI, USA), 0.5 lL of NP-40 and 2 lL of a fresh 1/24 dilution of stock primer mix.’’

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عنوان ژورنال:
  • PLoS Genetics

دوره 2  شماره 

صفحات  -

تاریخ انتشار 2006